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If making v2 sample sheets for devices in addition to the ones outlined, validate the ultimate sample sheet has the i5 index in the proper orientation as explained above.

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Explore the Illumina sequencing workflow in 3-dimensional depth. Go from sample planning to cluster technology, sequencing over a flow mobile with the proprietary SBS method, and info Assessment.

If working in manual mode and working with bcl2fastq or BCL Transform, or uploading the sample sheet to BaseSpace like a guide manner operate and attaching a sample sheet, enter the i5 sequence within the forward course.

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Established C Index Adapters Check with Index two (i5) Orientation To find out more on how to enter i5 bases on the sample sheet in ahead or reverse complement orientation.

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The application automatically makes the reverse complement as wanted for Investigation. When the sequence is reverse complemented, this could only be proven from the SampleSheetUsed.

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